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Original Research Article | OPEN ACCESS

Development of Reverse-Phase HPLC Method for Simultaneous Analysis of Metoprolol Succinate and Hydrochlorothiazide in a Tablet Formulation

Singh Brijesh2, D K Patel1, S K Ghosh2

1Analytical Chemistry Division, Indian Institute of Toxicology Research, M.G. Marg, Post Box 80, Lucknow; 2Department of Pharmaceutical Sciences, Dibrugarh University, Dibrugarh, Assam, India.

For correspondence:-  S Ghosh   Email: sghosh38@sancharnet.in   Tel:0373-2370254

Received: 9 February 2009        Accepted: 24 September 2009        Published: 21 December 2009

Citation: Brijesh S, Patel DK, Ghosh SK. Development of Reverse-Phase HPLC Method for Simultaneous Analysis of Metoprolol Succinate and Hydrochlorothiazide in a Tablet Formulation. Trop J Pharm Res 2009; 8(6):539-543 doi: 10.4314/tjpr.v8i6.9

© 2009 The authors.
This is an Open Access article that uses a funding model which does not charge readers or their institutions for access and distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0) and the Budapest Open Access Initiative (http://www.budapestopenaccessinitiative.org/read), which permit unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited..

Abstract

Purpose: To develop a simple, sensitive and rapid reverse phase HPLC method for the simultaneous analysis of metoprolol succinate and hydrochlorothiazide in a solid dosage form.
Methods: The drugs were analysed by a reverse phase C-18 column using 50mM di-sodium hydrogen phosphate:methanol:acetonitrile in a ratio of 525:225:250 as mobile phase. The flow rate was 1 ml/min and the compounds were detected by a UV-detector at 222 nm at a column temperature of 24 ± 2 ºC. The method was statistically validated for linearity and accuracy.
Results: The retention time and drug content of metoprolol succinate and hydrochlorothiazide were 5.38 min, 96.05 % and 3.04 min., 97.64 %, respectively.
Conclusion: The study shows that the developed method is simple and accurate and that it would be suitable for the simultaneous determination of metoprolol succinate and hydrochlorothiazide in pharmaceutical formulations.

Keywords: Metoprolol succinate; Hydrochlorothiazide; Simultaneous analysis; High performance liquid chromatography; Tablet formulation

Impact Factor
Thompson Reuters (ISI): 0.523 (2021)
H-5 index (Google Scholar): 39 (2021)

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